Protein Tools / BIAcore (SPR)
Symtera Analytics has expanded its analytical services to
include BIAcore. This powerful technology allows label – free study of both
large molecules, such as proteins, nucleic acids and carbohydrates, and small
molecules. In the heart of this technology is Surface Plasmon Resonance (SPR)
effect (please see the video on this page explaining its principles). Our lab
is equipped with several SPR instruments including the industry standard
BIAcore 3000 (General Electric Healthcare Life Sciences).
BIAcore technology can measure multiple parameters important
for pharmaceutical and biopharmaceutical researchers:
•
Protein/protein, protein/small molecule or
peptide interactions, nucleic acid interactions
•
Direct binding
–
kinetics of interaction – kon, koff
–
equilibrium binding - KD
•
Competitive binding assays – KD
•
Concentration measurements with and without
standards
•
Binding affinity with 10 pM<KD<1
mM,
•
Kinetics - kon <107M-1sec-1,
10-5sec-1<koff<1sec-1
It is a powerful tool to screen chemical libraries for the identification of new pharmaceuticals.
